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solubilization buffer ddm  (Anatrace)

 
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    Anatrace solubilization buffer ddm
    Solubilization Buffer Ddm, supplied by Anatrace, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ddm+solubilization+buffer/n+dodecyl+%CE%B2+d+maltopyranoside+ddm/pmc11341756-304-6-12
    Average 90 stars, based on 1 article reviews
    solubilization buffer ddm - by Bioz Stars, 2026-11
    90/100 stars

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    Incubation:

    Article Title: Structural basis for ligand promiscuity and high signaling activity of Kaposi's Sarcoma-associated Herpesvirus-encoded GPCR.
    Article Snippet: .. The resuspended mixture was then incubated with 100mL of 2× solubilization buffer (20mM HEPES pH 8.0, 100mM NaCl, 5mM MgCl2, 5mM imidazole, 2% sodium cholate, 0.1% n-Dodecyl-β-D-Maltoside (DDM, Anatrace, D310), 4 μL calf intestinal alkaline phosphatase (CIP, NEB M0525), 5mM 2-mercaptoethanol, 10 μM GDP) under nutation for 1 h. Following solubilization, the mixture was centrifuged again at 180,000× g for 30min, and the supernatant was incubated with NiNTA resin at 4 °C for 2 hwith rotation. ..

    other:

    Article Title: Allosteric competition and inhibition in AMPA receptors.
    Article Snippet: The supernatant was ultracentrifuged to pellet membranes (125,000g, 45 min), which were solubilized in solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n-dodecyl-β-d-maltopyranoside (DDM; Anatrace, D310)) and 0.2% cholesteryl hemisuccinate Tris salt (Anatrace, CH210) for 2 h at 4 °C under constant stirring.

    Article Title: Allosteric competition and inhibition in AMPA receptors
    Article Snippet: The supernatant was ultracentrifuged to pellet membranes (125,000 g , 45 min), which were solubilized in solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n -dodecyl-β- d -maltopyranoside (DDM; Anatrace, D310)) and 0.2% cholesteryl hemisuccinate Tris salt (Anatrace, CH210) for 2 h at 4 °C under constant stirring.

    Article Title: Architecture, Activation, and Conformational Plasticity in the GluA4 AMPA Receptor
    Article Snippet: Solubilization buffer (150mM NaCl, 20mM Tris pH 8.0, 1% n -dodecyl-β-D-maltopyranoside (DDM; Anatrace D310) and 0.2% Porcine Brain Total Lipid Extract (Avanti, 131101P)) was added to the pelleted membranes and membranes were solubilized for 2 hours at 4 °C in a cold room under constant agitation.

    Article Title: Structural insights into the allosteric inhibition of P2X4 receptors
    Article Snippet: The supernatant was collected after centrifugation at 8000 × g for 20 min and then ultracentrifuged at 200,000 × g for 1 h. The membrane fraction pellets were solubilized in solubilization buffer [50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2% n-dodecyl-beta-d-maltopyranoside (DDM) (Anatrace, USA)] with 1 mM PMSF, 5.2 μg/mL aprotinin, 2 μg/mL leupeptin, 1.4 μg/mL pepstatin A and 0.2 unit/mL apyrase (Sigma, USA), stirred for 2 h. The solubilized mixture was centrifuged at 200,000 × g for 1 h. The supernatant was loaded onto Strep-Tactin Superflow Plus beads (Qiagen, USA) pre-equilibrated with the wash buffer (100 mM Tris-HCl, pH 8.0, 150 mM NaCl and 0.05% DDM) and stirred for 1 h. The resin was washed with 10 CV of the wash buffer.

    Concentration Assay:

    Article Title: The translocation assembly module (TAM) catalyzes the assembly of bacterial outer membrane proteins in vitro
    Article Snippet: .. The resulting pellets were resuspended in Solubilization Buffer (PBS, 1% n-dodecyl-β-maltoside (DDM, Anatrace, catalog number D310), 37 mM imidazole, pH 7.4 at a concentration of 8–12 mL per liter of culture) and dispersed by a Dounce homogenizer. ..



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